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Tel:+44(01562)825286Fax:+44(01562)825284E-mail:info@severnbiotech.comWebsite:www.severnbiotech.comElectroBlottersTheV10-SDBandV20-SDBSemi-DryBlottersTheV10-SDB10x10cmandV20-SDB20x20cmsemi-dryblottersareidealforfasttransferofproteinsandnucleicacidswithouttheneedforcostlyaccessoriessuchasgelcassettesandtanks.Corrosion-freeplatinum-coatedtitaniumandstainlesssteelplateelectrodes,correspondingtotheanodeandcathode,maximisetheactiveareaoftransfersothatfullelectricalcontactismadebetweenthegelandthemembrane,allowingtransfertobecompletedwithinanhourwiththeminimumofheatdissipation,bufferconsumptionandmessinthelaboratory,normallyassociatedwithwetblottingtechniques.CompleteSystem10x10cmsemi-dryblotterV10-SDB20x20cmsemi-dryblotterV20-SDBTechnicalSpecificationV10-SDBV20-SDBUnitDimensions(WxDxH)17x7.5x17cm27x7.5x27cmActiveTransferArea(WxL)10x10cm20x20cmSampleThroughputOne10x10cmmini-gelFour10x10cmmini-gels;One20x20cmmaxi-gelRecommendedCurrentSetting80mA/0.8mAcm-2320mA/0.8mAcm-2PowerOutputConnectors4mm4mm(diameter)RecommendedPowerSuppliesEV243EV265,261&215BenefitsInclude.2sizesavailable:-.V10-SDB-10x10cmformat-accommodatesone10x10cmmini-gel.V20-SDB-20x20cmformat-accommodatesuptofour10x10cmmini-gelsorone20x20cmmaxi-gel.Gelscanbestacked-forhigherthroughputblotting.Rapidtransferassuredwithin1hour-forlowermolecularweightproteinsandnucleicacids;largermoleculestransferredin2hours.Semi-dryformat-minimisesbufferconsumption,withnoneedformessandadditionalaccessories.Colour-coded,corrosion-freeplateelectrodes-platinum-coatedtitaniumanodeandstainlesssteelcathodemaximisethetransferarea,sothatfullelectricalcontactismadebetweenthegelandthemembrane,whilecorrosionresistantmetalprolongslifespananddurabilitySemi-dryblottingwithSevernBiotechV10-SDBandV20-SDBunitsThreesheetsofblottingpaper,saturatedwithtransferbuffer,arefirstsuperimposedovertheanodeplate,followedbythemembrane,thegelandafurther3sheetsofblottingpaper.Thecathodeplateisthenplacedovertheblottingsandwichandthecurrentappliedat0.8mAcm-2for1to2hours.ElectroBlotters17ElectrophoresisEquipment

Tel:+44(01562)825286Fax:+44(01562)825284E-mail:info@severnbiotech.comWebsite:www.severnbiotech.comDot&SlotBlottersDot&slotmicrofiltrationmanifoldsavailableinalphanumeric96samplethroughputs,SevernBiotech'sdotandslotblotmicrofiltrationmanifoldsprovideaneasyandreproduciblemeanstohybridiseproteinsandnucleicacidsinsolutionontomembranes.Thesemanifolds,whicharemachinedfromhigh-densityacrylic,haveprecision-lappedmatingsurfacesthatensureuniformfilter/membranecontactwithoutgasketsand'O'rings.Manifoldassemblyissimple,beingachievedbythealignmentandtighteningofstainlesssteelthumbscrewsandmatingscrews,whilefilterscanbecutdowntotheexactsizeofthetemplate.TechnicalSpecificationDHM-96UnitDimensions(WxDxH)17x10.5x7cmMaximumSampleCapacity96DotDiameter3mmSlotDimensions(WxL)-SampleVolume/Dot100?lSampleVolume/Slot-MinimumMembraneSize(WxL)7.5x11.3cmVacuumPortExternalDiameter7mmVacuumPortInternalDiameter6mmMaximumOperatingTemperature65┬░CWorkingPressureofPump600mmHgorWaterAspirator(0.8Bar)CompleteSystem96-wellDotBlotHybridisationManifoldin8x12array,comprisingpairedupperdot-formingmodulesfordots3mmindiameter,andlowervacuumchambermodule.DHM-96BenefitsInclude.Rapidtransferassuredwithinminutes-dot/slotmicrofiltrationmanifoldallowsunfractionatedsamplestobeimmobilisedontoamembraneforimmediatescreeningwithnucleicacidprobesorantibodies.DHM-96-for7.5x11.3cmmembranes-96-samplethroughputVacuumreservoir-servesasacollectionchamberforfiltrateStainlesssteelthumbscrewsandprecision-lappedmatingsurfaces-maintainvacuumtransferwithoutsamplesmearing,crosscontaminationandleakageUppermanifold-allowssampletobeloadedintoeachdot/slotTemplate-allowsmembranestobecutdownsizeVacuumreservoir-servesasacollectionchamberforfiltrateDot&SlotBlotters18ElectrophoresisEquipment