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Tel:+44(01562)825286Fax:+44(01562)825284E-mail:info@severnbiotech.comWebsite:www.severnbiotech.comElectroBlottersTheTV100-EBKMiniandTV400-EBKMaxiElectroblottersRecentlyredesignedtoaccommodateupto4electroblottingcassettes,theSevernBiotechTV100-EBKandTV400-EBKelectroblottersprovideacost-effectiveandsafeandeasytousesystemforthetransferofproteinsandnucleicacidsfrom10x10cmminiand20.5x20cmmaxigels.Bothunitsaresuppliedwithasymmetriccolour-codedcassettesasauniquedesignfeaturetopreventthemfrombeinginsertedintothetankinthewrongorientation,againstthedirectionoftransfer.TechnicalSpecificationTV100-EBKTV400-EBKUnitDimensions(WxDxH)20x22.5x18.5cm19.5x32.5x28.5cmInnerTankDimensions(WxDxH)12.8x11x17cm12.8x21x26cmMaximumSampleCapacity4cassettes4cassettesRecommendedBufferVolume2000ml6300mlRecommendedRunningConditionsVoltage50-100VVoltage200VforElectroblottingCurrent75mA(0.6-0.8mA/cm2)Current150mA(0.8-1.0mA/cm2)Time2-4hTime6htoovernightDistanceBetweenPlateElectrodesinEBGRM12.8cm12.8cmElectroblottingCassetteDimensions(WxL)11.5x10.5cm20.5x20cmActiveTransferArea71cm2289cm2Snap-lockConnectorsforCoolingCoilInnerDiameter10mmInnerDiameter10mmOuterDiameter12mmOuterDiameter12mmQuick-fitTubingInnerDiameter10mmInnerDiameter10mmOuterDiameter12mmOuterDiameter12mmPowerOutputConnectors(diameter)Shrouded,4mmShrouded,4mmRecommendedPowerSuppliesConsortEV243ConsortEV243Snap-lockconnectorsforenhancedcoolingAsymmetriccolour-codedcassettesFibrepadsmaintainuniformcontactbetweenthegelandthemembraneCompleteSystemCompleteelectroblottingsystemforfour10x10cmmini-gels,including4xelectroblottingcassettes,4xfibrepads,cooledtank,lidandquick-fittubingTV100-EBKCompleteelectroblottingsystemforfour20.5x20cmmini-gels,including4xelectroblottingcassettes,4xfibrepads,cooledtank,lidandquick-fittubingTV400-EBKReplacementParts&Accessories1x(10x10cm)Hinged,colour-codedcassettewithfibrepadsTV100-EBC4x(10x10cm)FibrepadsEB-FPS1x(20.5x20cm)Hinged,colour-codedcassettewithfibrepadsTV400-EBC4x(20.5x20cm)FibrepadsEB-FPL2xQuick-fittubesforcoolingcoilTCS-CC2x0.2mmthick,platinumelectrodewirePT-0.22x1Metrepowerleadswithshrouded4mmpoweroutputconnectorsCABLE-4BenefitsIncludeAvailablein2differentformats:-.TV100-EBK-accommodatesamaximumoffour10x10cmelectroblottingcassettes.TV400-EBK-accommodatesamaximumoffour20.5x20cmelectroblottingcassettes.Asymmetric,colour-codedcassettes-hinged,withredandblackcoverscorrespondingtotheanodeandthecathode,toensurecorrecttransfer.Fibrepads-compressthegelagainstthenitrocellulosemembraneforuniformtransfer.Plate-electrodes-12.8cmapart-maximisetheintensityoftheelectricfield,allowingrapidtransferwithin4to6hours.Enhancedcooling-includedasstandard-snap-lockconnectorsallowthecoolingcoilwithineachunittobeconnectedtotheJULABOchillerunitsforfaster,high-intensitytransfers,orextendedovernightrunswithoutbufferdepletion.Flatbottom-allowseachelectroblottertobeplaceddirectlyontoamagneticstirringplateElectroBlotters16ElectrophoresisEquipment

Tel:+44(01562)825286Fax:+44(01562)825284E-mail:info@severnbiotech.comWebsite:www.severnbiotech.comElectroBlottersTheV10-SDBandV20-SDBSemi-DryBlottersTheV10-SDB10x10cmandV20-SDB20x20cmsemi-dryblottersareidealforfasttransferofproteinsandnucleicacidswithouttheneedforcostlyaccessoriessuchasgelcassettesandtanks.Corrosion-freeplatinum-coatedtitaniumandstainlesssteelplateelectrodes,correspondingtotheanodeandcathode,maximisetheactiveareaoftransfersothatfullelectricalcontactismadebetweenthegelandthemembrane,allowingtransfertobecompletedwithinanhourwiththeminimumofheatdissipation,bufferconsumptionandmessinthelaboratory,normallyassociatedwithwetblottingtechniques.CompleteSystem10x10cmsemi-dryblotterV10-SDB20x20cmsemi-dryblotterV20-SDBTechnicalSpecificationV10-SDBV20-SDBUnitDimensions(WxDxH)17x7.5x17cm27x7.5x27cmActiveTransferArea(WxL)10x10cm20x20cmSampleThroughputOne10x10cmmini-gelFour10x10cmmini-gels;One20x20cmmaxi-gelRecommendedCurrentSetting80mA/0.8mAcm-2320mA/0.8mAcm-2PowerOutputConnectors4mm4mm(diameter)RecommendedPowerSuppliesEV243EV265,261&215BenefitsInclude.2sizesavailable:-.V10-SDB-10x10cmformat-accommodatesone10x10cmmini-gel.V20-SDB-20x20cmformat-accommodatesuptofour10x10cmmini-gelsorone20x20cmmaxi-gel.Gelscanbestacked-forhigherthroughputblotting.Rapidtransferassuredwithin1hour-forlowermolecularweightproteinsandnucleicacids;largermoleculestransferredin2hours.Semi-dryformat-minimisesbufferconsumption,withnoneedformessandadditionalaccessories.Colour-coded,corrosion-freeplateelectrodes-platinum-coatedtitaniumanodeandstainlesssteelcathodemaximisethetransferarea,sothatfullelectricalcontactismadebetweenthegelandthemembrane,whilecorrosionresistantmetalprolongslifespananddurabilitySemi-dryblottingwithSevernBiotechV10-SDBandV20-SDBunitsThreesheetsofblottingpaper,saturatedwithtransferbuffer,arefirstsuperimposedovertheanodeplate,followedbythemembrane,thegelandafurther3sheetsofblottingpaper.Thecathodeplateisthenplacedovertheblottingsandwichandthecurrentappliedat0.8mAcm-2for1to2hours.ElectroBlotters17ElectrophoresisEquipment