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Tel:+44(01562)825286Fax:+44(01562)825284E-mail:info@severnbiotech.comWebsite:www.severnbiotech.comGradientMixersGradientMixersAvailablein15,25,50,100and500mlvolumes,SevernBiotech'sgradientmixersareidealforpouringpolyacrylamidegradientgelsandcentrifugationgradientsusingsucroseorcaesiumchloridesolutions.Eachgradientmixerisprecisionengineeredfromhighqualityacrylicplasticandcomprisesleak-freevalvesthatcontrolmixingbetweenthereservoirandmixingchambersandtheresultantoutputflow.MixingchamberOutletvalveReservoirchamberSupportrodMixingvalveBenefitsInclude.Availablein5differentsizes-fordifferentapplications.GM15andGM25-15and25mlvolumesforsucroseandcaesiumchloridegradients;theGM25canalsomaketwo1mmthick,10x10cmgradientgelsintheTV100-MC22-gelmulticaster.GM50-50mlvolumesufficienttopouruptofive1mmthick,10x10cmgradientgelsoronestandard1mmthick,20.5x20cmgelusingtheTV400castingbase.GM100-100mlvolumeforpouringuptoten1mmthick,10x10cmgelsintheTV100-MC1010-gelmulticasterortwo1mmthick,20.5x20cmgelsusingtheTV400-MC22-gelmulticaster.GM500-500mlvolumesuitableforuptoten1mmthick,20.5x20cmgelsintheTV400-MC1010-gelmulticaster.Reservoirchamber-designedtocontainhigherpercentageacrylamidesolutionwhenmakinggradientgelswiththeTV100andTV400multicasters.Mixingchamber-eachisofsufficientdiametertoaccommodateamagneticstirrertomixlowerpercentageacrylamidewithhigherpercentageacrylamidefromthereservoirchamber.Mixingchamber-eachisofsufficientdiametertoaccommodateamagneticstirrertomixlowerpercentageacrylamidewithhigherpercentageacrylamidefromthereservoirchamber.Valves-controloutputflowfromboththereservoirandmixingchambers.Flatbase-allowseachgradientmixertobeplacedontoamagneticstirringplate.Supportrod-secureseachgradientmixertoaretortstandCompleteSystem1x15mlGradientmixerGM151x25mlGradientmixerGM251x50mlGradientmixerGM501x100mlGradientmixerGM1001x500mlGradientmixerGM500TechnicalSpecificationGM15GM25GM50GM100GM500UnitDimensions(WxDxH)9x2.4x9cm10x2.4x11.5cm10x2.4x11.5cm11x3x13.5cm13x6x14cmInternalDimensionsofMixingandReservoir1.3x8cm1.4x10cm1.9x10cm2.5x12.5cm5.4x13cmChambers(DiameterxHeight)VolumeofEachChamber7.5ml12.5ml25ml50ml250mlInternalDiameterof2mm2mm2mm2mm5mmOutletPortVerticalGelUnits15ElectrophoresisEquipment

Tel:+44(01562)825286Fax:+44(01562)825284E-mail:info@severnbiotech.comWebsite:www.severnbiotech.comElectroBlottersTheTV100-EBKMiniandTV400-EBKMaxiElectroblottersRecentlyredesignedtoaccommodateupto4electroblottingcassettes,theSevernBiotechTV100-EBKandTV400-EBKelectroblottersprovideacost-effectiveandsafeandeasytousesystemforthetransferofproteinsandnucleicacidsfrom10x10cmminiand20.5x20cmmaxigels.Bothunitsaresuppliedwithasymmetriccolour-codedcassettesasauniquedesignfeaturetopreventthemfrombeinginsertedintothetankinthewrongorientation,againstthedirectionoftransfer.TechnicalSpecificationTV100-EBKTV400-EBKUnitDimensions(WxDxH)20x22.5x18.5cm19.5x32.5x28.5cmInnerTankDimensions(WxDxH)12.8x11x17cm12.8x21x26cmMaximumSampleCapacity4cassettes4cassettesRecommendedBufferVolume2000ml6300mlRecommendedRunningConditionsVoltage50-100VVoltage200VforElectroblottingCurrent75mA(0.6-0.8mA/cm2)Current150mA(0.8-1.0mA/cm2)Time2-4hTime6htoovernightDistanceBetweenPlateElectrodesinEBGRM12.8cm12.8cmElectroblottingCassetteDimensions(WxL)11.5x10.5cm20.5x20cmActiveTransferArea71cm2289cm2Snap-lockConnectorsforCoolingCoilInnerDiameter10mmInnerDiameter10mmOuterDiameter12mmOuterDiameter12mmQuick-fitTubingInnerDiameter10mmInnerDiameter10mmOuterDiameter12mmOuterDiameter12mmPowerOutputConnectors(diameter)Shrouded,4mmShrouded,4mmRecommendedPowerSuppliesConsortEV243ConsortEV243Snap-lockconnectorsforenhancedcoolingAsymmetriccolour-codedcassettesFibrepadsmaintainuniformcontactbetweenthegelandthemembraneCompleteSystemCompleteelectroblottingsystemforfour10x10cmmini-gels,including4xelectroblottingcassettes,4xfibrepads,cooledtank,lidandquick-fittubingTV100-EBKCompleteelectroblottingsystemforfour20.5x20cmmini-gels,including4xelectroblottingcassettes,4xfibrepads,cooledtank,lidandquick-fittubingTV400-EBKReplacementParts&Accessories1x(10x10cm)Hinged,colour-codedcassettewithfibrepadsTV100-EBC4x(10x10cm)FibrepadsEB-FPS1x(20.5x20cm)Hinged,colour-codedcassettewithfibrepadsTV400-EBC4x(20.5x20cm)FibrepadsEB-FPL2xQuick-fittubesforcoolingcoilTCS-CC2x0.2mmthick,platinumelectrodewirePT-0.22x1Metrepowerleadswithshrouded4mmpoweroutputconnectorsCABLE-4BenefitsIncludeAvailablein2differentformats:-.TV100-EBK-accommodatesamaximumoffour10x10cmelectroblottingcassettes.TV400-EBK-accommodatesamaximumoffour20.5x20cmelectroblottingcassettes.Asymmetric,colour-codedcassettes-hinged,withredandblackcoverscorrespondingtotheanodeandthecathode,toensurecorrecttransfer.Fibrepads-compressthegelagainstthenitrocellulosemembraneforuniformtransfer.Plate-electrodes-12.8cmapart-maximisetheintensityoftheelectricfield,allowingrapidtransferwithin4to6hours.Enhancedcooling-includedasstandard-snap-lockconnectorsallowthecoolingcoilwithineachunittobeconnectedtotheJULABOchillerunitsforfaster,high-intensitytransfers,orextendedovernightrunswithoutbufferdepletion.Flatbottom-allowseachelectroblottertobeplaceddirectlyontoamagneticstirringplateElectroBlotters16ElectrophoresisEquipment