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Tel:+44(01562)825286Fax:+44(01562)825284E-mail:info@severnbiotech.comWebsite:www.severnbiotech.comGradientGelElectrophoresisTheTV400-DGGEDenaturingGradientGelElectrophoresisSystemTheTV400-DGGEdenaturinggradientgelelectrophoresissystemisacost-effectivesolutionforresearchersstudyingmutationsandDNApolymorphismscriticalindiseaseaetiologyandgeneticdiversity.Designedprimarilyforparalleldenaturinggradientgelelectrophoresis(DGGE),whereelectrophoresisandthedenaturinggradientruninthesamedirection,theTV400-DGGEhasamaximum96-samplethroughputcompatiblewithstandardmicroplatesandthermalcyclerblocks.A100mlgradientmixerisalsoincludedtopourgradientgelsusingournewlydesignedgel-runningmoduleandcam-caster,whilea400Wheater,regulatedbyanexternaltemperaturecontrolunitconnectedtoaheatsensorwithinthegeltank,allowsthegeltemperaturetobesettothepredeterminedmeltingtemperature(Tm)ofthePCR®-amplifiedDNApolymorphismormutationofinterest.TechnicalSpecificationTV400GRM+HeatedTankUnitDimensions(WxDxH)28.5x15x29cmInnerTankDimensions(WxDxH)27x12.5x26cmPlateDimensions(WxHxT)20.5x20x0.4cmStandardSpacerDimensions(WxHxT)2x20x0.1cmActiveGelDimensions(WxH)2x24RecommendedBufferVolumeInnerBufferChamber700mlGelTank4200mlRecommendedRunningConditionsforVoltage175-225VDenaturing/NativePAGEGel(8.75-11.25v/cm)Current20-30mATime2.5-3.5hTemperatureRangeThermometer0-100°CPowerOutputofHeatingElement400WHeatingElementDimensions(LxD)14x1.5cmOperatingRangeofPT100TemperatureSensor-50to+200°CPT100TemperatureSensorDimensions(LxD)14x0.3cmCastingBaseSiliconeSealDimensions(WxLxH)1.7x22.5x0.8cmPowerOutputConnectors(diameter)Shrouded,4mmRecommendedPowerSuppliesConsortEV243HS-UNITUnitDimensions(WxDxH)9.6x14x8cmRecommendedTemperatureControlRange50to70°CTemperatureControlAccuracy+/-1°CGM100UnitDimensions(WxDxH)11x3x13.5cmInternalDimensionsofMixingandReservoirChambers(DiameterxHeight)2.5x12.5cmVolumeofEachChamber50mlInternalDiameterofOutletPort2mmCompleteSystemTwin-platemaxi-gelunitwithGRM,heatedgeltankwithPT100temperaturesensorandthermometer,lid,2x(20.5x20cm;WXH)plainglassplates,2x(20.5x20cm)notchedglassplates,4x1mmspacers,2xspaceralignersand2x1mmthick24-samplecombs,castingbase,2xsiliconesealsand1zdummyplate;plusGM100gradientmixerandheatsensorcontrolunitTV400-DGGEReplacementParts&Accessories1xTV400Gel-runningmoduleTV400-GRM2x(20.5x20cm)PlainglassplatesforTV400TV400-PG2x(20.5x20cm)NotchedglassplatesforTV400TV400-NG1xDummyplateTV400-DP2x1mmThickspacersforTV400TV400-S12xSpaceralignersforTV400TV400-SA1xCam-pincastingbasewith2siliconesealsTV400-CB2xSiliconesealsforcam-pincastingbaseTV400-CB-SEALS1x100mlGradientmixerGM1001xCastersystemfor2x(20.5x20cm)gradientmaxi-gelsTV400-MC21xCastersystemfor10x(20.5cm)gradientmaxi-gelsTV400-MC102x0.2mmThick,platinumelectrodewirePT-0.22x1Metrepowerleadswithshrouded4mmpoweroutputconnectorsCABLE-4Thickness(mm)ProductSampleThroughputToothWidth(mm)ToothHeight(mm)Max.Spacing(mm)SampleVolume/Well(?l)TV400-DGGETV400-C1-241244.7520295(standard)TV400-C1-481482.3520147(optional)GradientGelElectrophoresis14ElectrophoresisEquipment

Tel:+44(01562)825286Fax:+44(01562)825284E-mail:info@severnbiotech.comWebsite:www.severnbiotech.comGradientMixersGradientMixersAvailablein15,25,50,100and500mlvolumes,SevernBiotech'sgradientmixersareidealforpouringpolyacrylamidegradientgelsandcentrifugationgradientsusingsucroseorcaesiumchloridesolutions.Eachgradientmixerisprecisionengineeredfromhighqualityacrylicplasticandcomprisesleak-freevalvesthatcontrolmixingbetweenthereservoirandmixingchambersandtheresultantoutputflow.MixingchamberOutletvalveReservoirchamberSupportrodMixingvalveBenefitsInclude.Availablein5differentsizes-fordifferentapplications.GM15andGM25-15and25mlvolumesforsucroseandcaesiumchloridegradients;theGM25canalsomaketwo1mmthick,10x10cmgradientgelsintheTV100-MC22-gelmulticaster.GM50-50mlvolumesufficienttopouruptofive1mmthick,10x10cmgradientgelsoronestandard1mmthick,20.5x20cmgelusingtheTV400castingbase.GM100-100mlvolumeforpouringuptoten1mmthick,10x10cmgelsintheTV100-MC1010-gelmulticasterortwo1mmthick,20.5x20cmgelsusingtheTV400-MC22-gelmulticaster.GM500-500mlvolumesuitableforuptoten1mmthick,20.5x20cmgelsintheTV400-MC1010-gelmulticaster.Reservoirchamber-designedtocontainhigherpercentageacrylamidesolutionwhenmakinggradientgelswiththeTV100andTV400multicasters.Mixingchamber-eachisofsufficientdiametertoaccommodateamagneticstirrertomixlowerpercentageacrylamidewithhigherpercentageacrylamidefromthereservoirchamber.Mixingchamber-eachisofsufficientdiametertoaccommodateamagneticstirrertomixlowerpercentageacrylamidewithhigherpercentageacrylamidefromthereservoirchamber.Valves-controloutputflowfromboththereservoirandmixingchambers.Flatbase-allowseachgradientmixertobeplacedontoamagneticstirringplate.Supportrod-secureseachgradientmixertoaretortstandCompleteSystem1x15mlGradientmixerGM151x25mlGradientmixerGM251x50mlGradientmixerGM501x100mlGradientmixerGM1001x500mlGradientmixerGM500TechnicalSpecificationGM15GM25GM50GM100GM500UnitDimensions(WxDxH)9x2.4x9cm10x2.4x11.5cm10x2.4x11.5cm11x3x13.5cm13x6x14cmInternalDimensionsofMixingandReservoir1.3x8cm1.4x10cm1.9x10cm2.5x12.5cm5.4x13cmChambers(DiameterxHeight)VolumeofEachChamber7.5ml12.5ml25ml50ml250mlInternalDiameterof2mm2mm2mm2mm5mmOutletPortVerticalGelUnits15ElectrophoresisEquipment